NSF Org:	DBI Division of Biological Infrastructure
Recipient:	UNIVERSITY OF TEXAS AT AUSTIN
Initial Amendment Date:	September 22, 1998
Latest Amendment Date:	July 22, 1999
Award Number:	9808891
Award Instrument:	Continuing Grant
Program Manager:	Gerald Selzer DBI  Division of Biological Infrastructure BIO  Directorate for Biological Sciences
Start Date:	October 1, 1998
End Date:	September 30, 2001 (Estimated)
Total Intended Award Amount:	$147,252.00
Total Awarded Amount to Date:	$147,252.00
Funds Obligated to Date:	FY 1998 = $77,306.00 FY 1999 = $69,946.00
History of Investigator:	Jerry Brand (Principal Investigator) jbrand@mail.utexas.edu Kenneth Diller (Co-Principal Investigator)
Recipient Sponsored Research Office:	University of Texas at Austin 110 INNER CAMPUS DR AUSTIN TX  US  78712-1139 (512)471-6424
Sponsor Congressional District:	25
Primary Place of Performance:	University of Texas at Austin 110 INNER CAMPUS DR AUSTIN TX  US  78712-1139
Primary Place of Performance Congressional District:	25
Unique Entity Identifier (UEI):	V6AFQPN18437
Parent UEI:
NSF Program(s):	LIVING STOCK COLLECTIONS
Primary Program Source:	app-0198  app-0199
Program Reference Code(s):	HPCC, 9215, 9169
Program Element Code(s):	110600
Award Agency Code:	4900
Fund Agency Code:	4900
Assistance Listing Number(s):	47.074

ABSTRACT

9808891
Brand
This award renews support of efforts to define effective methods for cryopreservation of a large variety of single-celled algae (microalgae) including over 2000 strains found in the UTEX collection of freshwater algae directed by the principal investigator of this award. Such microalgae are used for basic research in genetics, ecology and physiology, and for varied commercial purposes. Development of reliable methods for their cryopreservation will eliminate the need for maintenance of strains through serial culture techniques. Serial culture techniques are costly and, as importantly, permit the genetic makeup of the strains to change over time, thereby limiting their usefulness for some types of research. Thus the development of reliable cryopreservation methods is of scientific as well as economic importance to the researchers and others who use microalgae. Progress to date suggests that well over 50% of the strains in the UTEX collection can be cryopreserved. Work supported through this work will allow completion of the testing of the collection and additional efforts aimed at development of new methods for dealing with those strains so far refractory to existing cryopreservation methodology.

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