NSF Org:	MCB Division of Molecular and Cellular Biosciences
Recipient:	UNIVERSITY OF OKLAHOMA
Initial Amendment Date:	July 10, 1995
Latest Amendment Date:	July 10, 1995
Award Number:	9509132
Award Instrument:	Standard Grant
Program Manager:	Marcia Steinberg MCB  Division of Molecular and Cellular Biosciences BIO  Directorate for Biological Sciences
Start Date:	July 15, 1995
End Date:	December 31, 1996 (Estimated)
Total Intended Award Amount:	$50,000.00
Total Awarded Amount to Date:	$50,000.00
Funds Obligated to Date:	FY 1995 = $50,000.00
History of Investigator:	Michelle Hanna (Principal Investigator) mmh@ribomed.com
Recipient Sponsored Research Office:	University of Oklahoma Norman Campus 660 PARRINGTON OVAL RM 301 NORMAN OK  US  73019-3003 (405)325-4757
Sponsor Congressional District:	04
Primary Place of Performance:	University of Oklahoma Norman Campus 660 PARRINGTON OVAL RM 301 NORMAN OK  US  73019-3003
Primary Place of Performance Congressional District:	04
Unique Entity Identifier (UEI):	EVTSTTLCEWS5
Parent UEI:
NSF Program(s):	MOLECULAR BIOCHEMISTRY
Primary Program Source:	app-0195
Program Reference Code(s):	9164, 9183, 9222, BIOT
Program Element Code(s):	116600
Award Agency Code:	4900
Fund Agency Code:	4900
Assistance Listing Number(s):	47.074

ABSTRACT

; R o o t E n t r y F ( C o m p O b j b W o r d D o c u m e n t O b j e c t P o o l 6X 6X + ! " # $ % & ' ( ) * , - . / 0 1 2 3 4 5 6 7 8 9 : ; < = > ? @ A B C D E F G H I F Microsoft Word 6.0 Document MSWordDoc Word.Document.6 ; e = e * * n n n n n n n > 1 o T ; > n > n n n n n n n n 4 9509132 Hanna This project uses biophysical techniques to analyze the molecular mechanisms involved in the regulation of gene expression at the level of transcription, which is catalyzed by RNA polymerase. The work is focused primarily on E. coli, which is an ideal model system in which mechanistic studies can be carried out using purified components. Several novel ribonucleotide analogs which are tagged with photocrosslinking, fluorescent, or other tags have been developed in this laboratory. These will be used for analysis of protein-nucleic acid interactions in the E. coli transcription complex, using electron microscopy and fluorescence polarization techniques %%% Interactions between proteins and nucleic acids are important in many cellular functions, including the regulation of gene expression. This project uses biophysical techniques to study how the enzyme RNA polymerase, which is a protein, attaches to the DNA of a bacterium and transcribes the information in the DNA into RNA, which then becomes the blueprint for protein synthesis. *** Oh +' 0 S u m m a r y I n f o r m a t i o n ( $ H l D h R:\WWUSER\TEMPLATE\NORMAL.DOT 9509132 marcia steinberg marcia steinberg @ 2 @ @ 2 @ Microsoft Word 6.0 1 ; * & * ! ! ! ! ! K @ Normal a b c " A@ " Default Paragraph Font * * * * 4 marcia steinberg \\CLM11\MCBPUB\BIOCHEM\HANNA.ABS @HP DeskJet 500 LPT1: HPDSKJET HP DeskJet 500 D L f , , ! /' ^ }w;/' } d g 2 HP DeskJet 500 D L f , , ! /' ^ }w;/' } d g 2 % % % $ 1 Times New Roman Symbol & Arial " h v\ v\ $#; 9509132 marcia steinberg marcia steinberg ;

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