NSF Org:	MCB Division of Molecular and Cellular Biosciences
Recipient:	UNIVERSITY OF TEXAS AT AUSTIN
Initial Amendment Date:	April 12, 1989
Latest Amendment Date:	August 11, 1992
Award Number:	8819169
Award Instrument:	Continuing Grant
Program Manager:	Philip Harriman MCB  Division of Molecular and Cellular Biosciences BIO  Directorate for Biological Sciences
Start Date:	May 1, 1989
End Date:	October 31, 1992 (Estimated)
Total Intended Award Amount:	$307,584.00
Total Awarded Amount to Date:	$307,584.00
Funds Obligated to Date:	FY 1989 = $100,855.00 FY 1990 = $96,200.00 FY 1991 = $110,529.00
History of Investigator:	Shelley Payne (Principal Investigator) payne@mail.utexas.edu
Recipient Sponsored Research Office:	University of Texas at Austin 110 INNER CAMPUS DR AUSTIN TX  US  78712-1139 (512)471-6424
Sponsor Congressional District:	25
Primary Place of Performance:	DATA NOT AVAILABLE
Primary Place of Performance Congressional District:
Unique Entity Identifier (UEI):	V6AFQPN18437
Parent UEI:
NSF Program(s):	Genetic Mechanisms, CROSS-DIRECTORATE PROGRAMS, SPECIAL PROGRAMS-RESERVE
Primary Program Source:
Program Reference Code(s):	9232, 9251, 9268
Program Element Code(s):	111200, 912000, 914500
Award Agency Code:	4900
Fund Agency Code:	4900
Assistance Listing Number(s):	47.074

ABSTRACT

Iron is an essential, but elusive element for most cells, and high affinity transport systems are usually required for its acquisition. The long range goals of this project are to characterize the regulation of the iron acquisition systems and other iron regulated genes of the enteric bacterial genus, Shigella. These studies will include (1) characterizing the mechanisms of regulation of enterobactin expression in Shigella flexneri, (2) measuring the expression of iron-regulated genes in an in vivo system, and (3) characterizing the positive and negative regulatory elements which affect expression of the gene for Congo red/heme binding (crb). Experimental approaches will utilize genetic and biochemical techniques. In vivo expression of the genes will be investigated using fusions to reporter genes, such as lux, whose expression can be readily monitored, and an in vivo labeling system has been developed to compare proteins synthesized by bacteria inside host cells to those observed in vitro. Characterization of mutants with altered gene regulation is also proposed. These studies will help define mechanisms of gene regulation both in vivo and in vitro.

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