NSF Org:	IOS Division Of Integrative Organismal Systems
Recipient:	UNIVERSITY OF NOTRE DAME DU LAC
Initial Amendment Date:	March 17, 1988
Latest Amendment Date:	March 17, 1988
Award Number:	8718178
Award Instrument:	Standard Grant
Program Manager:	Vincent P. Gutschick IOS  Division Of Integrative Organismal Systems BIO  Directorate for Biological Sciences
Start Date:	April 15, 1988
End Date:	September 30, 1989 (Estimated)
Total Intended Award Amount:	$50,000.00
Total Awarded Amount to Date:	$50,000.00
Funds Obligated to Date:	FY 1988 = $50,000.00
History of Investigator:	Frederick Goetz (Principal Investigator) rick@uwm.edu
Recipient Sponsored Research Office:	University of Notre Dame 940 GRACE HALL NOTRE DAME IN  US  46556-5708 (574)631-7432
Sponsor Congressional District:	02
Primary Place of Performance:	DATA NOT AVAILABLE
Primary Place of Performance Congressional District:
Unique Entity Identifier (UEI):	FPU6XGFXMBE9
Parent UEI:	FPU6XGFXMBE9
NSF Program(s):	PHYSIOLOG & STRUCTURAL SYS
Primary Program Source:
Program Reference Code(s):
Program Element Code(s):	114100
Award Agency Code:	4900
Fund Agency Code:	4900
Assistance Listing Number(s):	47.074

ABSTRACT

Past research implicates eicosanoids, the phosphatidylinositol/protein kinase C system and proteolytic enzymes in fish ovulation. The proposal research will increase our understanding of the role of these three mediators in fish ovulation. Specifically, the production of lipoxygense metabolites by brook trout and goldfish ovaries will be investigated using radio labeled precursor incorporation, followed by metabolite separation on high performance liquid chromatography. The production of primary prostaglandins during steroid-induced ovulation in yellow perch will be investigated using radioimmunoassay. An incorporation system, involving the labeling of follicular phosphatidylinositol by radioactive myoinositol, will be investigated so that future research can use this system to study the cycling of follicular inositol phosphates following agonist stimulation. Total follicular proteolytic activity thoughout maturation and ovulation will be followed in brook trout and goldfish using substrate polyacrylamide gel electrophoresis. Collagenolytic activity will be followed through ovulation in brook trout follicles using a specific substrate assay to determine the role of this proteases in the release of the eggs from the follicles. The goal is a better understanding of the endocrine processes regulating ovulation in fish.

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