| NSF Org: |
CBET Division of Chemical, Bioengineering, Environmental, and Transport Systems |
| Recipient: |
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| Initial Amendment Date: | September 11, 2017 |
| Latest Amendment Date: | September 11, 2017 |
| Award Number: | 1726095 |
| Award Instrument: | Standard Grant |
| Program Manager: |
Shahab Shojaei-Zadeh
sshojaei@nsf.gov (703)292-8045 CBET Division of Chemical, Bioengineering, Environmental, and Transport Systems ENG Directorate for Engineering |
| Start Date: | October 1, 2017 |
| End Date: | September 30, 2021 (Estimated) |
| Total Intended Award Amount: | $289,328.00 |
| Total Awarded Amount to Date: | $289,328.00 |
| Funds Obligated to Date: |
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| History of Investigator: |
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| Recipient Sponsored Research Office: |
3640 COLONEL GLENN HWY DAYTON OH US 45435-0002 (937)775-2425 |
| Sponsor Congressional District: |
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| Primary Place of Performance: |
OH US 45435-0001 |
| Primary Place of
Performance Congressional District: |
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| Unique Entity Identifier (UEI): |
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| Parent UEI: |
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| NSF Program(s): | Major Research Instrumentation |
| Primary Program Source: |
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| Program Reference Code(s): |
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| Program Element Code(s): |
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| Award Agency Code: | 4900 |
| Fund Agency Code: | 4900 |
| Assistance Listing Number(s): | 47.041 |
ABSTRACT
This project involves acquisition of a CytoViva optical microscope instrument that will benefit over 10 faculty and 100 undergraduate and graduate students (including underrepresented groups) at three Midwestern institutions of higher education, namely Wright State University (WSU), Otterbein University, Ohio, and Ball State University (BSU), Indiana. The cutting-edge equipment will facilitate the optical imaging and spectral confirmation of a wide range of nanomaterials in live biological systems or composite materials. Thereby, its acquisition will strengthen the research of these scientists and will lead to new interdisciplinary collaborations that will offer fundamental insight into critical nano-biomedical and environmental problems. The exploitation of the CytoViva system as a teaching tool will contribute to the development of modern academic curricula and the next generation of instrumentalists. Furthermore, over 100 high school students will be annually engaged in hands-on nano-related activities (Exploring STEMM Program at WSU). The scientific results and pedagogic materials will be shared with the scientific community at large and general public through various venues.
The proposed CytoViva system will consist of a high signal-to-noise-enhanced dark-field optical microscope and an integrated hyperspectral imaging system. It will be equipped with a proprietary Dual Fluorescence Module, which will facilitate the real time, simultaneous imaging of both fluorescently-labeled and non-labeled nanomaterials with high spectral. The CytoViva technique has the following unique capabilities: a) no sample preparation and no alteration in the targeted environment, b) improved time- and cost-efficiency, c) pixel-specific spectral data, and d) the capability of differentiating between similar nanostructures based on finite differences in their aggregation state, orientation, and functionalization. The breadth of interdisciplinary research projects impacted by the CytoViva system will include a) identifying the broad-spectrum antiviral mechanism of silver nanoparticles against deadly viruses, b) developing methodologies for studying the cellular trafficking and transformations of noble metal nanomaterials for biomedical applications, c) understanding the molecular basis of gene expression regulation involved in the antibiotic resistance of pathogenic bacteria using gold nanoparticles functionalized with aptamers, d) treating and detecting docetaxel resistant prostate cancer by targeted nanomedical formulation, and e) cytosolic mapping of digitalis Na/K-ATPase complex internalization as a potentially new cellular signaling mechanism.
PUBLICATIONS PRODUCED AS A RESULT OF THIS RESEARCH
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PROJECT OUTCOMES REPORT
Disclaimer
This Project Outcomes Report for the General Public is displayed verbatim as submitted by the Principal Investigator (PI) for this award. Any opinions, findings, and conclusions or recommendations expressed in this Report are those of the PI and do not necessarily reflect the views of the National Science Foundation; NSF has not approved or endorsed its content.
Summary: A CytoViva Hyperspectral Imaging (CHSI) system was purchased and successfully installed at Wright State University (WSU) with the help of this NSF-MRI Award. The CHSI system is designed for the optical observation and hyperspectral confirmation of nanostructures and other materials present in fixed or living organisms and composite materials.
Intellectual Merit: Important knowledge gaps in several multidisciplinary nanoscience and biomedical research projects at Midwestern institutions were addressed through the routine use of the CHSI system. One of these projects demonstrated that CHSI could be employed as a pharmacokinetic tool for studying the health concerns associated with the biomedical applications of silver nanoparticles (AgNPs) involving the cardiovascular system such as antimicrobial agents and drug carriers. For example, AgNPs capped with a citrate biocompatible agent were found to strongly interact with the membrane of human red blood cells (RBCs), to cause membrane injury, and to disrupt the Na+/K+ pump. Two-dimensional (2-D) and 3-D CHSI revealed that over 70% of the interacting AgNPs were distributed at the membrane level. A CHSI library was created based on the hyperspectral signatures characteristic to controls and reference biochemical compounds making up the RBC membrane and the cytoplasmic matrix. A spectral analysis process was developed to extract for the first time not only qualitative but also quantitative information about the molecular binding mechanism of AgNPs to cells using the CHSI library. The high percent uptake (~48-65 ±5%) and strong interactions of AgNPs with human RBCs suggest that these citrate-capped AgNPs could remain concentrated in the circulatory system.
Broader Impacts: The NSF-MRI Award facilitated the recruitment, education, and training of over 50 high school, undergraduate, and graduate students under the principal investigator (PI)'s and co-PI's supervision alone. This resulted into seven graduate theses in Chemistry, Environmental Sciences, and Biomedical Engineering, four peer-reviewed manuscripts, 14 conference presentations, several prestigious awards and scholarships (e.g., the Outstanding Oral Presentation Award from the Chemical Toxicology section of the 2021 Meeting of the American Chemical Society, and the West Rock Scholarship at WSU). The CHSI facility was toured during Open House events, CytoViva workshops, and the Exploring STEMM Summer Program at WSU, which annually engages over 150 students in hands-on activities that illustrate the synthesis and applications of nanomaterials.
Last Modified: 10/02/2021
Modified by: Ioana E Pavel
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