| NSF Org: |
CBET Division of Chemical, Bioengineering, Environmental, and Transport Systems |
| Recipient: |
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| Initial Amendment Date: | December 17, 2009 |
| Latest Amendment Date: | March 12, 2010 |
| Award Number: | 0965545 |
| Award Instrument: | Continuing Grant |
| Program Manager: |
Aleksandr Simonian
asimonia@nsf.gov (703)292-2191 CBET Division of Chemical, Bioengineering, Environmental, and Transport Systems ENG Directorate for Engineering |
| Start Date: | July 1, 2009 |
| End Date: | August 31, 2010 (Estimated) |
| Total Intended Award Amount: | $0.00 |
| Total Awarded Amount to Date: | $12,467.00 |
| Funds Obligated to Date: |
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| History of Investigator: |
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| Recipient Sponsored Research Office: |
1960 KENNY RD Columbus OH US 43210-1016 (614)688-8734 |
| Sponsor Congressional District: |
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| Primary Place of Performance: |
1960 KENNY RD COLUMBUS OH US 43210-1016 |
| Primary Place of
Performance Congressional District: |
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| Unique Entity Identifier (UEI): |
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| Parent UEI: |
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| NSF Program(s): | Cellular & Biochem Engineering |
| Primary Program Source: |
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| Program Reference Code(s): |
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| Program Element Code(s): |
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| Award Agency Code: | 4900 |
| Fund Agency Code: | 4900 |
| Assistance Listing Number(s): | 47.041 |
ABSTRACT
David W. Wood
BES-0348220
The objective of this proposed study is to develop a new family of ligand-triggered self-splicing and self-cleaving proteins with a wide variety of potential applications. In practice, the switch proteins will be fused to various target proteins at the DNA level to generate functionally inactive precursors. The binding of a small molecule ligand by the engineered switch protein will trigger the switch to splice or cleave as desired for a particular application, typically resulting in the activation of the fused target protein. The development of these protein switches will yield insights into the design and behavior of artificial multifunctional proteins, particularly in the area of allosteric domain fusions, and will include aspects of rational as well as evolution-based approaches. Preliminary results imply immediate uses for these protein switches in biosensing and drug discovery, and new technologies in bioseparations are forthcoming. Long term applications include drug delivery, metabolic engineering and even biomolecular computation. The proposed pedagogical materials seek to provide students with information and skills to allow them to make greater contributions at the interface between engineering and biology, and include a high school intern program, the use of learning style evaluations in an introductory engineering course and a seminar series in applied biotechnology.
PUBLICATIONS PRODUCED AS A RESULT OF THIS RESEARCH
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