Award Abstract # 0954578
CAREER: Multiplex microfluidic and automation tools for neurogenetics and live imaging
| NSF Org: |
CBET
Division of Chemical, Bioengineering, Environmental, and Transport Systems
|
| Recipient: |
GEORGIA TECH RESEARCH CORP
|
| Initial Amendment Date:
|
December 2, 2009 |
| Latest Amendment Date:
|
December 2, 2009 |
| Award Number: |
0954578 |
| Award Instrument: |
Standard Grant |
| Program Manager: |
Leon Esterowitz
CBET
Division of Chemical, Bioengineering, Environmental, and Transport Systems
ENG
Directorate for Engineering
|
| Start Date: |
January 1, 2010 |
| End Date: |
August 31, 2016 (Estimated) |
| Total Intended Award
Amount: |
$400,518.00 |
| Total Awarded Amount to
Date: |
$400,518.00 |
| Funds Obligated to Date:
|
FY 2010 = $400,518.00
|
| History of Investigator:
|
-
Hang
Lu
(Principal Investigator)
hang.lu@chbe.gatech.edu
|
| Recipient Sponsored Research
Office: |
Georgia Tech Research Corporation
926 DALNEY ST NW
ATLANTA
GA
US
30318-6395
(404)894-4819
|
| Sponsor Congressional
District: |
05
|
| Primary Place of
Performance: |
Georgia Institute of Technology
225 NORTH AVE NW
ATLANTA
GA
US
30332-0002
|
Primary Place of
Performance
Congressional District: |
05
|
| Unique Entity Identifier
(UEI): |
EMW9FC8J3HN4
|
| Parent UEI: |
EMW9FC8J3HN4
|
| NSF Program(s): |
BioP-Biophotonics
|
| Primary Program Source:
|
01001011DB NSF RESEARCH & RELATED ACTIVIT
|
| Program Reference
Code(s): |
004E,
014E,
1045,
1187,
9102
|
| Program Element Code(s):
|
723600
|
| Award Agency Code: |
4900
|
| Fund Agency Code: |
4900
|
| Assistance Listing
Number(s): |
47.041
|
ABSTRACT
0954578
Lu
The long-term objective is to develop and use powerful microfluidic and automation tools to understand genetic pathways that regulate the biochemical communications between neurons and other tissues in C. elegans. Microfluidics is ideal for studies of small organisms such as C. elegans because of the relevant length scales and the possibility of integration and automation. The research objective of the CAREER project is to engineer a microfluidic system for live imaging of dynamic processes in vivo, to accomplish automated image processing, and to identify roles of genes in neuronal biochemical communications.This system will significantly increase the throughput and accuracy of in vivo live imaging experiments in model organisms. It streamlines and automates the painstakingly manual procedure of microscopy, in some cases enables some experiments that are otherwise impossible to do, and reduces the noise and artifacts in these experiments. The image analysis algorithms will provide quantitative data with large throughput to allow good statistics. The approach is innovative because the technologies developed here dramatically increase the capabilities and throughput of current assay tools, enabling key biological experiments that are not currently performed. Furthermore, the technology is broadly applicable to other biological systems and could potentially lead to new therapeutics for related diseases.
PUBLICATIONS PRODUCED AS A RESULT OF THIS RESEARCH
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(Showing: 1 - 10 of 24)
(Showing: 1 - 24 of 24)
33.Mei Zhan, Matthew M. Crane, Eugeni Entchev, Antonio Caballero, Diana Andrea Fernandes de Abreu, QueeLim Ch?ng, and Hang Lu
"Automated Processing of Imaging Data Through Multi-Tiered Classification of Biological Structures Illustrated Using Caenorhabditis elegans"
PLoS Computational Biology
, 2015
Daniel C. Williams, Rachid El Bejjani, Paula Mugno Ramirez, Sean Coakley, Shinae Kim, Hyewon Lee, Quan Wen, Aravi Samuel, Hang Lu, Massimo A. Hilliard, Marc Hammarlund
"Rapid and permanent neuronal inactivation in vivo via subcellular generation of reactive oxygen with the use of KillerRed"
Cell reports
, 2013
doi: 10.1016/j.celrep.2013.09.023
Diana Andrea Fernandes de Abreu, Antonio Caballero, Pascal Fardel, Nicholas Stroustrup, Zhunan Chen, KyungHwa Lee, William D. Keyes, Zachary M. Nash, Isaac F. López Moyado, Federico Vaggi, Astrid Cornils, Martin Regenass, Anca Neagu, Ivan Ostojic, Chang L
"An Insulin-to-Insulin Regulatory Network Orchestrates Phenotypic Specificity in Development and Physiology"
PLoS Genetics
, v.10
, 2014
, p.e1004225
Eugeni V. Entchev*, Dhaval S. Patel*, Mei Zhan*, Andrew J. Steele, Hang Lu, and QueeLim Ch'ng
"A gene-expression-based neural code for food abundance that modulates lifespan"
eLife
, v.4
, 2015
, p.e06259
Eugeni V. Entchev*, Dhaval S. Patel*, Mei Zhan*, Andrew J. Steele, Hang Lu+, and QueeLim Ch?ng
"A gene-expression-based neural code for food abundance that modulates lifespan"
eLife
, 2015
DOI: http://dx.doi.org/10.7554/eLife.06259
Guillaume Aubry and Hang Lu
"A perspective on optical developments in microfluidic platforms for Caenorhabditis elegans research"
biomicrofluidics
, v.8
, 2014
, p.11301
Guillaume Aubry and Hang Lu
"A perspective on optical developments in microfluidic platforms for Caenorhabditis elegans research"
Biomicrofluidics
, 2014
doi: 10.1063/1.4865167
Guillaume Aubry, Mei Zhan, and Hang Lu
"Hydrogel-droplet microfluidic platform for high-resolution imaging and sorting of early larval Caenorhabditis elegans"
Lab Chip
, v.15
, 2015
, p.1424
10.1039/C4LC01384K
Hyewon Lee, Matthew M. Crane, Yun Zhang, and Hang Lu
"Quantitative screening of genes regulating tryptophan hydroxylase transcription in C. elegans using microfluidics and adaptive algorithm"
Integrative Biology
, 2013
doi: 10.1039/c2ib20078c
Hyewon Lee, Shin Ae Kim, Paula Mugno, Marc Hammarlund, Massimo A. Hilliard, and Hang Lu
"A Multi-channel device for high-density target-selective stimulation and long-term monitoring of cells and subcellular features in C. elegans"
Lab Chip
, 2014
DOI: 10.1039/C4LC00789A
Hyewon Lee, Shin Ae Kim, Paula Mugno, Marc Hammarlund, Massimo A. Hilliard, and Hang Lu
"A Multi-channel device for high-density target-selective stimulation and long-term monitoring of cells and subcellular features in C. elegans"
Lab on a Chip
, v.14
, 2014
10.1039/C4LC0078
Hyundoo Hwang, Jan Krajniak, Yohei Matsunaga, Guy Benian, and Hang Lu
"On-demand optical immobilization of Caenorhabditis elegans for high-resolution imaging and microinjection"
Lab Chip
, 2014
DOI: 10.1039/C4LC00697F
Hyundoo Hwang, Jan Krajniak, Yohei Matsunaga, Guy Benian, and Hang Lu
"On-demand optical immobilization of Caenorhabditis elegans for high-resolution imaging and microinjection"
Lab on a chip
, v.14
, 2014
, p.3498
Ivan de Carlos Cáceres, Nicholas Valmas, Massimo A. Hilliard, Hang Lu
"Laterally Orienting C. elegans Using Geometry at Microscale for High-Throughput Visual Screens in Neurodegeneration and Neuronal Development Studies"
PloS one
, 2012
doi:10.1371/journal.pone.0035037
Jan Krajniak and Hang Lu
"Long-term High-Resolution Imaging and Culture of C. elegans in Chip-Gel Hybrid Microfluidic Device for Developmental Studies"
Lab Chip
, 2010
DOI:10.1039/c001986k
Jan Krajniak, Hang Lu
"Long-term High-Resolution Imaging and Culture of C. elegans in Chip-Gel Hybrid Microfluidic Device for Developmental Studies"
Lab on a Chip
, v.10
, 2010
, p.1862
DOI:10.1039/c001986k
Jan Krajniak, Yan Hao, Ho Yi Mak, Hang Lu
"CLIP ? Continuous Live Imaging Platform for Direct Observation of C. elegans Physiological Processes"
Lab Chip
, 2013
DOI:10.1039/C3LC50300C
Jan Krajniak, Yan Hao, Ho Yi Mak, Hang Lu
"CLIP ? Continuous Live Imaging Platform for Direct Observation of C. elegans Physiological Processes"
Lab on a Chip
, v.13
, 2013
, p.2963
DOI:10.1039/C3LC50300C
Kwanghun Chung*, Mei Zhan*, Jagan Srinivasan, Paul W. Sternberg, Emily Gong, Frank Schroeder, and Hang Lu
"Microfluidic chamber arrays for whole-organism behavior-based chemical screening"
Lab Chip
, 2011
DOI:10.1039/C1LC20400A
Mei Zhan, Loice Chingozha, and Hang Lu
"Enabling Systems Biology Approaches Through Microfabricated Systems"
Analytical Chemistry
, 2013
doi: 10.1021/ac401472y
Peri T. Kurshan, Allan Q. Phan, George J. Wang, Matthew M. Crane, Hang Lu, Kang Shen
"Regulation of synaptic extracellular matrix composition is critical for proper synapse morphology"
J. Neurosci
, v.34
, 2014
, p.12678-126
Xiaoni Ai, Weipeng Zhuo, Qionglin Liang, Patrick T. McGrath, and Hang Lu
"A high-throughput device for size based separation of C. elegans developmental stages"
Lab on a Chip
, v.14
, 2014
, p.1746
Xiaoni Ai, Weipeng Zhuo, Qionglin Liang+, Patrick T. McGrath+, and Hang Lu
"A high-throughput device for size based separation of C. elegans developmental stages"
Lab Chip
, 2014
doi: 10.1039/c3lc51334c.
(Showing: 1 - 10 of 24)
(Showing: 1 - 24 of 24)
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