Award Abstract # 0928249
Collaborative Research: Coupled Thermal-Hydrological-Mechanical-Chemical-Biological Experimental Facility at DUSEL Homestake

NSF Org: CMMI
Division of Civil, Mechanical, and Manufacturing Innovation
Recipient: BARNARD COLLEGE
Initial Amendment Date: August 14, 2009
Latest Amendment Date: June 14, 2011
Award Number: 0928249
Award Instrument: Continuing Grant
Program Manager: Richard Fragaszy
CMMI
 Division of Civil, Mechanical, and Manufacturing Innovation
ENG
 Directorate for Engineering
Start Date: August 15, 2009
End Date: July 31, 2013 (Estimated)
Total Intended Award Amount: $66,001.00
Total Awarded Amount to Date: $66,001.00
Funds Obligated to Date: FY 2009 = $24,724.00
FY 2010 = $24,861.00

FY 2011 = $16,416.00
History of Investigator:
  • Brian Mailloux (Principal Investigator)
    bjm2103@columbia.edu
Recipient Sponsored Research Office: Barnard College
3009 BROADWAY
NEW YORK
NY  US  10027-6909
(212)854-2708
Sponsor Congressional District: 13
Primary Place of Performance: Barnard College
3009 BROADWAY
NEW YORK
NY  US  10027-6909
Primary Place of Performance
Congressional District:
13
Unique Entity Identifier (UEI): LPQ1NHRK78M9
Parent UEI:
NSF Program(s): Instrumentation & Facilities,
GEOMECHANICS & GEOMATERIALS
Primary Program Source: 01000910DB NSF RESEARCH & RELATED ACTIVIT
01001011DB NSF RESEARCH & RELATED ACTIVIT

01001112DB NSF RESEARCH & RELATED ACTIVIT
Program Reference Code(s): 025E, 037E, 043E, 1057, CVIS
Program Element Code(s): 158000, 163400
Award Agency Code: 4900
Fund Agency Code: 4900
Assistance Listing Number(s): 47.041

ABSTRACT

This project will develop a preliminary design and work-breakdown-structure for a large-scale subsurface experimental facility to investigate coupled thermal-hydrological-mechanical-chemical-biological processes in fractured rock at depth. The experiment will be part of the proposed Deep Underground Science and Engineering Laboratory (DUSEL) in the Homestake Mine, South Dakota. Many natural and engineered earth systems involve coupling of multiple processes in rocks that vary across a wide range of scales. The most pervasive process in the Earth?s crust that gives rise to strongly coupled phenomena is the flow of fluids (water, CO2, hydrocarbons, magmas) through fractured heated rock under stress. Understanding changes in the reactivity, deformability, life-supporting and transport properties of rocks that fluids infiltrate is important in a broad range of geological engineering and geological science endeavors. Despite this fundamental importance, the interactions remain poorly understood.

The project will: (1) Determine properties of Homestake rocks: geological, geochemical, mechanical, thermal, isotopic, and reactivity. (2) Upscale these data to elucidate transport mechanisms (conductive versus convective), natural reaction rates in fractures, and microbial community evolution. (3) Evaluate monitoring strategies, in-situ probes and sampling methods, and necessary measurements. (4) Select a candidate site for the evaluating coupled processes. (5) Develop a work-breakdown-structure. (6) Develop a coupled numerical model to evaluate potential effects on the rock mass and optimal heater configuration, power, and monitoring borehole orientations.

The models and insight from these experiments will have broad applicability to engineered systems, e.g., enhanced geothermal systems, CO2 sequestration and subsurface contaminant transport. Educational outreach will involve facility tours and a traveling benchscale ?mock-up? demonstration experiment.

PUBLICATIONS PRODUCED AS A RESULT OF THIS RESEARCH

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Mailloux, Brian J. Dochenetz, Audra Bishop, Michael Dong, Hailiang Ziolkowski, Lori A. Wommack, K. Eric Sakowski, Eric G. Onstott, Tullis C. Slater, Greg F. "A carbon free filter for collection of large volume samples of cellular biomass from oligotrophic waters" Journal of Microbiological Methods , v.90 , 2012 , p.145 10.1016/j.mimet.2012.04.016
Mailloux, Brian J.Dochenetz, AudraBishop, MichaelDong, HailiangZiolkowski, Lori A.Wommack, K. EricSakowski, Eric G.Onstott, Tullis C.Slater, Greg F. "A carbon free filter for collection of large volume samples of cellular biomass from oligotrophic waters" Journal of Microbiological Methods , v.90 , 2012 , p.145 10.1016/j.mimet.2012.04.016

PROJECT OUTCOMES REPORT

Disclaimer

This Project Outcomes Report for the General Public is displayed verbatim as submitted by the Principal Investigator (PI) for this award. Any opinions, findings, and conclusions or recommendations expressed in this Report are those of the PI and do not necessarily reflect the views of the National Science Foundation; NSF has not approved or endorsed its content.

The goal of this work was to develop a method to improve microbial sampling of subsurface environments in order to understand the controls on life in extreme environments.  In particular we wanted to sample the carbon isotopes of cell wall material to determine the sources of carbon for microbial growth.  What is the source of carbon for subsurface organisms?  In particular we focused on the of Phospholipid fatty acids (PLFAs) of the microbial cells walls.  In order to sample and extract the PLFA’s it requires a filter that is free of organic carbon contamination.  We designed and built a filter made from aluminum coated glass wool in a stainless steel holder.  The filter was designed and tested as part of an undergraduate based class at Barnard College.  The filter is baked at 450°C for 24 hours to remove contaminants before deployment.  The system was utilized in the Gold Mines of South Africa during initial testing and we were able to filter over 4000 gallons while it was left unattended for 4 days.  Ample material was collected for radiocarbon analysis of PLFAs.  Initial results are showing a diverse range of carbon utilized by the microorganisms in this environment.  The filter is now being utilized for other projects including sampling of groundwater wells.  


Last Modified: 11/20/2013
Modified by: Brian J Mailloux

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