Award Abstract # 0623400
In-Situ Classification of Bloom-Forming Phytoplankton by Imaging Multivariate Optical Computing (IMOC)

NSF Org: OCE
Division Of Ocean Sciences
Recipient: SOUTH CAROLINA RESEARCH FOUNDATION
Initial Amendment Date: August 4, 2007
Latest Amendment Date: August 4, 2007
Award Number: 0623400
Award Instrument: Standard Grant
Program Manager: Kandace Binkley
kbinkley@nsf.gov
 (703)292-7577
OCE
 Division Of Ocean Sciences
GEO
 Directorate for Geosciences
Start Date: August 1, 2007
End Date: July 31, 2010 (Estimated)
Total Intended Award Amount: $514,498.00
Total Awarded Amount to Date: $514,498.00
Funds Obligated to Date: FY 2007 = $514,498.00
History of Investigator:
  • Timothy Shaw (Principal Investigator)
    shaw@mail.chem.sc.edu
  • Michael Myrick (Co-Principal Investigator)
  • Tammi Richardson (Co-Principal Investigator)
  • Benjamin Twining (Co-Principal Investigator)
Recipient Sponsored Research Office: University South Carolina Research Foundation
915 BULL ST
COLUMBIA
SC  US  29208-4009
(803)777-7093
Sponsor Congressional District: 06
Primary Place of Performance: University of South Carolina at Columbia
1600 HAMPTON ST
COLUMBIA
SC  US  29208-3403
Primary Place of Performance
Congressional District:
06
Unique Entity Identifier (UEI): ELBVJ1KYX976
Parent UEI:
NSF Program(s): OCEAN TECH & INTERDISC COORDIN,
BE: INSTRUM DEVELOP FOR ENV AC
Primary Program Source: app-0107 
Program Reference Code(s): 0000, 9150, OTHR
Program Element Code(s): 168000, 169400
Award Agency Code: 4900
Fund Agency Code: 4900
Assistance Listing Number(s): 47.050

ABSTRACT

The PIs propose to develop a simple system for the in situ identification and detection of three major phytoplankton bloom species in real time. The system will be based on simultaneous analysis of their size and their fluorescence excitation spectral characteristics using a new method called imaging multivariate optical computing (IMOC). This method will produce an optical ""bar code"" to discriminate between phytoplankton taxa. IMOC works by imaging through an optical filter uniquely designed for spectral analysis for a specific application such as phytoplankton discrimination. It requires that the investigators develop a database of phytoplankton fluorescence excitation spectra under varying growth conditions and employ canonical discriminant analysis to identify one or more discriminant functions to identify phytoplankton taxa.

The PIs will use IMOC to combine instant spectroscopic analysis with a streak-camera-type microscopic imaging system to provide real-time or near-real-time classification. The method is ideal for field deployable instrumentation, making it an ideal addition to ocean observing platforms.

Broader Impacts

Identification of marine phytoplankton is a critical area in biological oceanography. Successful implementation of this research could go far towards lowering the ambiguities in the identification of marine phytoplankton, which could easily be automated for use from buoys and other remote platforms. An educational outreach program will be pursued through an existing program (""Rising Tide"") that links high school teachers and undergraduate interns in summer research projects. Interns from South Carolina HBCU institutions would be targeted for this experience. Commercial development/private sector partnerships with academia would likely result from a successful project.

PUBLICATIONS PRODUCED AS A RESULT OF THIS RESEARCH

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L.S. Hill, T.L. Richardson, L.T.M. Profeta, T.J. Shaw, C.J. Hintz, B.S. Twining, E. Lawrenz, M.L. Myrick "Construction, Figures Of Merit And Testing Of A Single-Cell Fluorescence Excitation Spectroscopy System" Review of Scientific Instruments , v.81 , 2010 , p.013103-1

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