NSF Org:	OCE Division Of Ocean Sciences
Recipient:	WOODS HOLE OCEANOGRAPHIC INSTITUTION
Initial Amendment Date:	August 18, 2004
Latest Amendment Date:	October 13, 2006
Award Number:	0350712
Award Instrument:	Standard Grant
Program Manager:	Kandace Binkley kbinkley@nsf.gov  (703)292-7577 OCE  Division Of Ocean Sciences GEO  Directorate for Geosciences
Start Date:	September 1, 2004
End Date:	August 31, 2007 (Estimated)
Total Intended Award Amount:	$0.00
Total Awarded Amount to Date:	$150,000.00
Funds Obligated to Date:	FY 2004 = $150,000.00
History of Investigator:	Robert Olson (Principal Investigator) rolson@whoi.edu Heidi Sosik (Co-Principal Investigator)
Recipient Sponsored Research Office:	Woods Hole Oceanographic Institution 266 WOODS HOLE RD WOODS HOLE MA  US  02543-1535 (508)289-3542
Sponsor Congressional District:	09
Primary Place of Performance:	Woods Hole Oceanographic Institution 266 WOODS HOLE RD WOODS HOLE MA  US  02543-1535
Primary Place of Performance Congressional District:	09
Unique Entity Identifier (UEI):	GFKFBWG2TV98
Parent UEI:
NSF Program(s):	OCEAN TECH & INTERDISC COORDIN
Primary Program Source:	app-0104
Program Reference Code(s):	0000, OTHR
Program Element Code(s):	168000
Award Agency Code:	4900
Fund Agency Code:	4900
Assistance Listing Number(s):	47.050

ABSTRACT

The PIs propose is to test the feasibility of developing a LIBS flow cytometer for the study of plankton populations. Ultimately the goal would be to integrate a multi-laser flow cytometer a high spectral resolution fiber optic LIBS system. Initially they will assemble a skeleton flow cytometer from components in their lab to be combined with a commercial LIBS system. Experiments will be set up to answer the following questions: 1) will the flow cell be damaged by a LIBS laser pulse powerful enough to generate the required plasma? 2) do bubbles from the plasma generation disrupt the flow so severely as to prevent subsequent measurements? and 3) can the LIBS system detect single phytoplankton cells above background seawater signals? The results will determine how to proceed with the next phase.

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