| NSF Org: |
CBET Division of Chemical, Bioengineering, Environmental, and Transport Systems |
| Recipient: |
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| Initial Amendment Date: | April 30, 2001 |
| Latest Amendment Date: | June 25, 2003 |
| Award Number: | 0120690 |
| Award Instrument: | Continuing Grant |
| Program Manager: |
Fred G. Heineken
CBET Division of Chemical, Bioengineering, Environmental, and Transport Systems ENG Directorate for Engineering |
| Start Date: | May 1, 2001 |
| End Date: | April 30, 2005 (Estimated) |
| Total Intended Award Amount: | $249,999.00 |
| Total Awarded Amount to Date: | $249,999.00 |
| Funds Obligated to Date: |
FY 2002 = $83,295.00 FY 2003 = $86,814.00 |
| History of Investigator: |
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| Recipient Sponsored Research Office: |
21 N PARK ST STE 6301 MADISON WI US 53715-1218 (608)262-3822 |
| Sponsor Congressional District: |
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| Primary Place of Performance: |
21 N PARK ST STE 6301 MADISON WI US 53715-1218 |
| Primary Place of
Performance Congressional District: |
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| Unique Entity Identifier (UEI): |
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| Parent UEI: |
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| NSF Program(s): | Cellular & Biochem Engineering |
| Primary Program Source: |
app-0102 app-0103 |
| Program Reference Code(s): |
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| Program Element Code(s): |
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| Award Agency Code: | 4900 |
| Fund Agency Code: | 4900 |
| Assistance Listing Number(s): | 47.041 |
ABSTRACT
The secretion of heterologous proteins that contain disulfide bonds is not at its full potential. Evidence suggests that the regulation of the redox state of the endoplasmic reticulum may be a bottleneck. A multifaceted approach is proposed to probe and manage the redox state in yeast in order to investigate the bottleneck hypothesis further and to ultimately increase secretion efficiency. The four elements of the proposed work are: (1) amplify the products of the individual genes now thought to be involved redox management to assess the effect, (2) use display methods to uncover possibly missing pathway elements, (3) add redox altering compounds to the growth medium to alter redox state, and (4) develop a tachyplesin I substrate-based diagnostic system.
PUBLICATIONS PRODUCED AS A RESULT OF THIS RESEARCH
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