
NSF Org: |
DEB Division Of Environmental Biology |
Recipient: |
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Initial Amendment Date: | March 15, 2016 |
Latest Amendment Date: | February 12, 2024 |
Award Number: | 1556645 |
Award Instrument: | Continuing Grant |
Program Manager: |
Matthew Herron
mherron@nsf.gov (703)292-5361 DEB Division Of Environmental Biology BIO Directorate for Biological Sciences |
Start Date: | June 1, 2016 |
End Date: | May 31, 2025 (Estimated) |
Total Intended Award Amount: | $1,064,000.00 |
Total Awarded Amount to Date: | $1,064,000.00 |
Funds Obligated to Date: |
FY 2018 = $309,758.00 FY 2019 = $208,947.00 |
History of Investigator: |
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Recipient Sponsored Research Office: |
1600 HAMPTON ST COLUMBIA SC US 29208-3403 (803)777-7093 |
Sponsor Congressional District: |
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Primary Place of Performance: |
715 Sumter Street Columbia SC US 29208-0001 |
Primary Place of
Performance Congressional District: |
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Unique Entity Identifier (UEI): |
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Parent UEI: |
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NSF Program(s): |
Evolutionary Processes, EVOLUTIONARY GENETICS, Dimensions of Biodiversity |
Primary Program Source: |
01001819DB NSF RESEARCH & RELATED ACTIVIT 01001920DB NSF RESEARCH & RELATED ACTIVIT 01002021DB NSF RESEARCH & RELATED ACTIVIT |
Program Reference Code(s): |
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Program Element Code(s): |
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Award Agency Code: | 4900 |
Fund Agency Code: | 4900 |
Assistance Listing Number(s): | 47.074 |
ABSTRACT
This research seeks to determine how often genetic mutations occur, whether they produce a change in how gene activity levels respond to the environment, and whether those altered responses have effects on observable traits. This study will provide insight into how genetic differences among organisms arise, the basis for all evolutionary change and the diversity of life. Differences among organisms are driven, in part, by differences in their genes and differences in the rate at which genetic information is used for the building of proteins and other cell structures, the activity of those genes. Genetic differences arise due to errors made when copying DNA, the molecule in which genetic information is stored. If these errors happen during cell divisions that lead to an individual's offspring, the differences will be inherited by all future generations. A parallel educational activity will develop a computer-based application that simulates copying errors in the context of music. When students learn genetics, they often begin with several misconceptions about mutations. However, realistic experiments illustrating properties of mutations are difficult to execute in class settings. Therefore, the project includes development of "Mutational Music", an application for mobile devices that uses computer-based music to simulate spontaneous mutations, along with an educational program appropriate for use in introductory genetics laboratories.
Spontaneous mutations are the ultimate source of genetic variation; understanding their characteristics is therefore a fundamental goal of biologists. Existing work has quantified the rate and effects of spontaneous mutations, usually focusing on the molecular spectrum of mutations or on fitness effects. These foci are widely separated, and the processes that link molecular mutations to fitness effects have received less attention. This project is aimed at bridging the gap between fitness and the molecular spectrum of mutations, focusing on mutational changes in the transcriptome and in phenotypic plasticity of key life history traits. The research involves a mutation accumulation experiment in the model crustacean Daphnia pulex, coupled with analyses of gene expression and life history traits made in distinct resource environments. The work will generate estimates of the mutational variance and heritability of expression for each gene, including the ability to identify allele-specificity and splice variant-specificity. In addition, the work will quantify the distribution of effects spontaneous mutations have on life history traits and their plasticity. The components will be joined to investigate the potential that expression mutations contribute to the origin of phenotypic plasticity.
PUBLICATIONS PRODUCED AS A RESULT OF THIS RESEARCH
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