| NSF Org: |
CBET Division of Chemical, Bioengineering, Environmental, and Transport Systems |
| Recipient: |
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| Initial Amendment Date: | April 25, 2016 |
| Latest Amendment Date: | April 25, 2016 |
| Award Number: | 1637863 |
| Award Instrument: | Standard Grant |
| Program Manager: |
Suk-Wah Tam-Chang
stamchan@nsf.gov (703)292-8684 CBET Division of Chemical, Bioengineering, Environmental, and Transport Systems ENG Directorate for Engineering |
| Start Date: | August 1, 2016 |
| End Date: | July 31, 2019 (Estimated) |
| Total Intended Award Amount: | $150,314.00 |
| Total Awarded Amount to Date: | $150,314.00 |
| Funds Obligated to Date: |
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| History of Investigator: |
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| Recipient Sponsored Research Office: |
310 E CAMPUS RD RM 409 ATHENS GA US 30602-1589 (706)542-5939 |
| Sponsor Congressional District: |
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| Primary Place of Performance: |
310 East Campus Rd. Athens GA US 30602-1589 |
| Primary Place of
Performance Congressional District: |
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| Unique Entity Identifier (UEI): |
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| Parent UEI: |
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| NSF Program(s): | BIOSENS-Biosensing |
| Primary Program Source: |
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| Program Reference Code(s): |
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| Program Element Code(s): |
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| Award Agency Code: | 4900 |
| Fund Agency Code: | 4900 |
| Assistance Listing Number(s): | 47.041 |
ABSTRACT
PI Ramasamy, Ramaraja P.
Proposal No 1637863
Rapid and early detection of pathogens during food processing operations is strategic for preventing contamination, improving food safety, quality and hygiene. The proposed project would provide a near-ideal solution for this identified problem.
Rapid and early detection of pathogens in food processing operations is strategic for preventing contamination, improving food safety, quality and hygiene. The proposed project will develop a near-ideal solution to this problem. A novel method for simultaneous isolation (enrichment) and detection of a foodborne pathogen, Listeria monocytogenes, from complex food matrices using bacteriophage as bio-recognition entity will be characterized. Because available antibodies for Listeria monocytogenes have poor avidity, the PI will genetically engineer bacteriophage for this specific purpose. Specific research objectives are: (1) isolate L. monocytogenes by magnetic separation from model food matrix using recombinant A511 phage. (2) Demonstrate rapid detection of L. monocytogenes on A511 modified electrodes using AC impedance. Education and outreach objectives are to broaden participation of women in engineering, promote diversity in STEM disciplines and provide interdisciplinary education for engineering undergraduate students.
PUBLICATIONS PRODUCED AS A RESULT OF THIS RESEARCH
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PROJECT OUTCOMES REPORT
Disclaimer
This Project Outcomes Report for the General Public is displayed verbatim as submitted by the Principal Investigator (PI) for this award. Any opinions, findings, and conclusions or recommendations expressed in this Report are those of the PI and do not necessarily reflect the views of the National Science Foundation; NSF has not approved or endorsed its content.
The project titled 'A novel method for simultaneous enrichment and detection of bacterial cells' was focussed on developing a multi-step method for rapid diagnosis of bacterial cells with particular focus on the identification of Listeria monocytogenes in food samples. More specifically we have developed a bacteriophage based method for both rapid separation and isolation of bacterial cells from food matrices as well as quantitative detection of the isolated cells using an electrochemical biosensing platform. We have acheived through surface modification of electrodes as well as magnatic microparticles using a bacteriophage virus that selectively binds to the host cells without killing them. the binding between the phage and the bacteria results in the generation of an electrochemical signal at the electrode that can be quantified to determine the number of cells in the sample. The methods has been tested using simulated food samples as well as artifically contaminated milk and ground beef with Listeria sp. The intellectual merit of the proposed project lies in the development of a new methodology for detection bacterial cells that could be used for any application. The results from this project offer a viable pathways for the development of new test methods of rapid diagnosis of food pathogen infections that does not require a traditional molecular biology tools and therefore have a broader impact in food and healthcare industry.
Last Modified: 12/23/2019
Modified by: Ramaraja P Ramasamy
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